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Bài 1 Phân tích Coombs test – Phan Trúc
นอกจากการดูบทความนี้แล้ว คุณยังสามารถดูข้อมูลที่เป็นประโยชน์อื่นๆ อีกมากมายที่เราให้ไว้ที่นี่: ดูเพิ่มเติม
Đây là bài mở đầu của chuỗi bài giảng về chủ đề Ngân hàng máu, được thực hiện bởi nhóm Diễn đàn bác sĩ trẻ Việt Nam (VYPO Vietnam Young Physician Organization). Mọi người nhớ theo dõi page để cập nhật nhiều khoá học bổ ích và hoàn toàn miễn phí nhé.
Group: https://www.facebook.com/groups/diendanbacsitrevietnam
Facebook cá nhân: https://www.facebook.com/drtrucphan
Website: https://vypo.ykhoa.org
Kỹ thuật làm Coombs gián tiếp
The Antiglobulin Tests
Developed and produced for http://www.MechanismsInHematology.com by Mechanisms in Medicine Inc.
Animation description: The Antiglobulin Tests (also known as Coombs test). The direct antiglobulin test (DAT) is requested when immunemediated hemolytic anemia is suspected. A blood sample must be drawn.
The patient’s red blood cells may have antibodies (and other complement system factors) on the surface of some of the red blood cells (abbreviated as RBCs in this animation).
The blood sample is washed to remove the patient’s plasma while leaving the red blood cells intact with their attached antibodies.
These red blood cells are incubated with antihuman globulin containing both antiIgG and
anticomplement.
The antihuman globulin binds to the patient’s antibody, coating the red blood cells, resulting in agglutination.
A positive DAT indicates that the red cells have been coated in vivo with immunoglobulin or complement and may suggest the presence of an
immunemediated hemolytic process. The DAT is used in the investigation of transfusion reactions, autoimmune hemolytic anemia, hemolytic disease of the newborn and druginduced hemolysis. Rarely the DAT may be positive in a normal individual.
To detect whether human antired blood cell antibodies are present in the plasma of a patient, an indirect antiglobulin test (IAT) is used.
Unlike the DAT which relies on the red blood cells in the sample, the IAT only deals with the extracted plasma.
Unbound human antired blood cell antibodies remain in the plasma.
The plasma is then incubated with reagent red blood cells selected to detect specific antibodies that are considered clinically significant.
The antired blood cell antibodies of the patient’s plasma bind to reagent red blood cells to form antigenantibody complexes.
The addition of antihuman globulin to these complexes results in agglutination of the red blood cells. A positive IAT indicates the presence of a red cell antibody in the patient.
Direct Coombs Test
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Kỹ thuật huyết thanh Coombs
Huyet hoc
นอกจากการดูหัวข้อนี้แล้ว คุณยังสามารถเข้าถึงบทวิจารณ์ดีๆ อื่นๆ อีกมากมายได้ที่นี่: ดูบทความเพิ่มเติมในหมวดหมู่GENERAL NEWS
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