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RNA Extraction by TRIzol® | เว็บนี้ให้ข่าวสารล่าสุดทุกวัน.
รูปภาพที่เกี่ยวข้องกับหัวข้อ RNA Extraction by TRIzol®.
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ความรู้ที่เกี่ยวข้องกับบทความ การสกัด rna
http://www.abnova.com ) – TRIzol® เป็นสารละลายโมโนฟาซิกของฟีนอลและกัวนิดีน ไอโซไทโอไซยาเนต เป็นรีเอเจนต์พร้อมใช้งานสำหรับการแยก RNA ทั้งหมด …
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แท็กเกี่ยวข้องกับบทความการสกัด rna.
#RNA #Extraction #TRIzol
[vid_tags]RNA Extraction by TRIzol®
การสกัด rna.
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@0:09 to the sample… which sample????? are all samples treated the same way as in here??? @2:02 wash pellet… where is the pellet??? @1:56 what is happening exactly here?? emptying all the content??? why why the music!!! Speak instead, give instructions !!!
Why we incubate with ice?? To prevent degradation Of RNA??
After adding isopropanol and centrifuging it, could you see a white clear pellet? Please help
Thank y very much
music is obnoxious.
Great video, it is very useful, we are procuer of guanidine thiocyante, we can give some advise to our customer after learn this very ,thank you very much.
thanks
the dnase treatment should be before or after the phenol chloroform
I am using Trizol method for total RNA isolation from plant leaf. From my result, the nano drop reads 260/230 and 260/280 is equal 2.163 and 2.00 respectively. The quality on gel was shown no distinct bands! The RNA is degraded. Actually my sample is not fresh sample and was stored on deep(-80 degree C)freeze more than six months. what is the causes of RNA degradation? Is degraded RNA can be used for cDNA synthesis for qRT-PCR analysis?
Why why why the fucking disturbing music!!! why!!!
Can I use the same protocol for fungal spores without liquid N2?
can i use the same protocol for plant material
By these method IAM getting 3 bands In gel but OD is coming 1.3-1.5 what is the problem……?
I am using 200 mg of sample for 2 ml trizol I get thick pellet but it gets dislodge after ethanol wash and sometimes decant with the ethanol please suggest what should I do that it doesn't get dislodge from the bottom?
One question why the sample with trizol and chloroform was incubated on ice not room temperature? I always do on room temperature
Thank you, this is really a very helpful guide to the protocol that comes along with the TriZol reagent.
When I do the same procedure, I obtain 3 phases after the chloroform and triazol, is it correct? It is a sample from adipose tissue.
Thanks a lot!!! 😀
This technique is really great !!!!!!! Loved the video 🙂
Kubota centrifuge. Like the most famous flip flops. Very nice music.
If I air dry RNA until the pellet become transparent, is it going to affect the concentration of the RNA or the result?
This extraction can be done from a whole blood sample in EDTA.
Thank you so much I did the same as demo…
you need to have RNase Inhibitor in the end. 1 ul for every 30 ul of water.
Thanks so much.
Good tune lol
gud one………….
You need at least -80 degree fridge for RNA medium-long term storage.
"Hey can you tell me if this rag smells like chloroform?"
I hope that you're using a hood when using chemicals like trizol and chloroform! :O
thank you,it was really useful.
was the sample treated with liquid N2?
Thank you for your help!! 😀 Happy Easter
The best if you make the reverse transcription,then store the sample,because the RNA is really sensitive.
Just a short question.. after the extraction can I put the RNA in the -4°C and use it when I need it? Or it will compromise the RNA? Thanks!!
@BrianK0220 you get an alcohol reaction which may damage the RNA